Comparative Studies on Caseins of Several Different Animals by Polyacrylamide Gel Electrophoresis
نویسندگان
چکیده
منابع مشابه
Polyacrylamide Gel Electrophoresis
Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...
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MATERIALS 5x TBE electrophoresis buffer Polyacrylamide gels are poured and run in 0.5x or 1x TBE at low voltage (1-8 V/cm) to prevent denaturation of small fragments of DNA by Joulic heating. Other electrophoresis buffers such as 1x TAE (please see Agarose Gel Electrophoresis) can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much...
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The application of a method of electrophoretic protein analysis to the investigation of the total protein composition of Salmonella typhimurium is described. It involves electrophoresis of many samples simultaneously on a thin slab of acrylamide gel, in the presence of sodium dodecyl sulfate, with the discontinuous buffer system of Laemmli ((19’70) Nature 227, 680485). Up to about 25 samples ca...
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D ISCOT1XUOUS ELECT1IO1’HORESIS in polyacrylamide rods has been used for separation of serum proteins (1). In spite of satisfactory resolution in tile rod, the technic renhains cumbersome, and comparison of the closely migrating cOnlpoflents inaccurate. Akroyd (2) reported discontinuous electrophoresis On polyacrylamide gel slab, which alleviates many of the difficulties of the rod. We llave co...
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Twenty-three control samples of CSF were analyzed by vertical column electrophoresis on acrylamide gel. Quantitation results using a microdensitometer are presented. The normal protein pattern shows 15 constant bands which coincide in mobility and in many cases in staining pattern with serum proteins. The haptoglobin peaks, except for the band of the 1-1 type and the leading band of the 2-1 typ...
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ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1970
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.44.505